水稻SSR及Indel分子标记在籼粳交F2群体的偏分离分析

doi:10.3969/j.issn.1006-8082.2014.05.003

摘要/Abstract

摘要： 偏分离是指遗传标记的观察频率偏离预期的孟德尔式分离频率的现象，其可以增加群体中杂合等位基因或者异型染色体的频率，是一种重要的进化动力。本研究以粳稻材料日本晴及籼稻材料千粒稻为亲本获得80株F2群体，构建了一张含92对SSR标记和44对Indel标记的遗传图谱。结果发现，F2群体中，116个标记表现为偏分离现象（85.29%），其中79个极度偏分离（68.10%）。偏分离标记中由合子偏分离引起的有20个，配子偏分离引起的93个，RM3484，RM3702，os7-38.4既不是由合子选择引起也不是由配子选择引起。Indel标记偏分离率（97.72%）明显高于SSR标记偏分离率（79.34%），且所有偏分离标记皆偏向于千粒稻。结合本研究结果，对偏分离现象产生的原因进行了分析。

关键词: SSR, Indel, 偏分离, F2群体, 水稻

Abstract: Segregation distortion refers to the phenomenon observed deviations from the expected frequency of genetic markers of Mendelian frequency separation, which has attracted considerable attention as an evolutionary force that can cause an increase in the population frequency of the heterozygous allele or heteromorphic chromosome.In this study,a rice genetic linkage map comprising 92 SSR and 44 Indel markers loci was constructed using an F2 population consisting of 80 lines from a sub-specific cross between japonica rice Nipponbare and indica rice Qianlidao. The results showed that F2 population was significantly distorted. Among these markers, 116 markers showed segregation distortion（85.29%）, 79 markers extreme showed segregation distortion（68.10%）. Segregation distortion caused by the choice of the zygote has 20, gamete select caused 93, RM3484, RM3702, os7-38.4 is neither caused by a homozygous select nor gametes select. Segregation distortion rate for Indel markers（97.72%） were significantly higher than that for SSR markers（79.34%）. All marks deviated preference to paternal Qianlidao. The causes of segregation distortion were discussed based on the results and previous report.

Key words: SSR, indel, segregation distortion, F2, rice

中图分类号:

S511

张向阳1,张红宇1,徐培洲1,陈晓琼1,蒋彬1,韩弥鹏1,陈钰堂1,王志2,黄廷友2,吴先军1*. 水稻SSR及Indel分子标记在籼粳交F2群体的偏分离分析[J]. 中国稻米, 2014, 20(5): 13-17.

参考文献

[1]    Harushima Y, Kurata N, Yano M, et al. Detection of segregation distortions in an indica-japonica rice cross using a high-resolution molecular map[J]. Theor Appl Genet, 1996, 92（2）: 145-150.

[2]    Lu H, Romero-Severson J, Bernardo R.Chromosomal regions associated with segregation distortion in maize[J]. Theor Appl Genet, 2002, 105（4）: 622-628.

[3]    Mangelsdorf P C, Jones D F. The expression of Mendelian factors in the gametophyte of maize[J]. Genetics, 1926, 11（5）: 423.

[4]    Li W M, Tang D Z, Wu W R, et al. A molecular map based on an indica/indica recombinant inbred population and its comparison with an existing map derived from indica/japonica cross in rice[J]. Chinese J Rice Sci, 2000, 14（2）: 71-78.

[5]    Yang J P, Rong T Z, Huang L J, et al. Construction of the frame molecular linkage map in maize[J]. Acta Agronomica Sinica, 2004, 30（1）: 82-87.

[6]    Konishi T, Yano Y, Abe K. Geographic distribution of alleles at the Ga2 locus for segregation distortion in barley[J]. Theor Appl Genet, 1992, 85（4）: 419-422.

[7]    刘刚，许盛宝，倪中福，等. 小麦 RIL 群体 SSR 标记偏分离的遗传分析[J].农业生物技术学报，2007，15（5）：828-833.

[8]    刘峰，吴晓雷，陈受宜. 大豆分子标记在 RIL 群体中的偏分离分析[J]. 遗传学报，2000，27（10）：883-887.

[9]    肖炳光，徐照丽，陈学军，等. 利用 DH 群体构建烤烟分子标记遗传连锁图[J]. 中国烟草学报，2006，12（4）：35-40.

[10] 梁爽. 利用 InDel 和 SSR 整合大白菜分子遗传图谱[D]. 郑州：郑州大学，2013.

[11] 王茂芊，李博，王华忠. 甜菜遗传连锁图谱初步构建[J]. 作物学报, 2014，40（2）：222-230.

[12] Lespinasse D, Rodier-Goud M, Grivet L, et al. A saturated genetic linkage map of rubber tree （Hevea spp.） based on RFLP, AFLP, microsatellite, and isozyme markers[J]. Theor Appl Genet, 2000, 100（1）: 127-138.

[13] Tai G C C, Seabrook J E A, Aziz A N. Linkage analysis of anther-derived monoploids showing distorted segregation of molecular markers[J]. Theor Appl Genet, 2000, 101（1-2）: 126-130.

[14] 刘中来，李军，瞿邵洪. 利用RFP标记进行水稻籼粳杂种偏分离的遗传分析[J]. 浙江农业学报，2012，24（2）：189-192

[15] Lorieux M, Goffinet B, Perrier X, et al. Maximum-likelihood models for mapping genetic markers showing segregation distortion. 1. Backcross populations[J]. Theor Appl Genet, 1995, 90（1）: 73-80.

[16] Lorieux M, Perrier X, Goffinet B, et al. Maximum-likelihood models for mapping genetic markers showing segregation distortion. 2. F2 populations[J]. Theor Appl Genet, 1995, 90（1）: 81-89.

[17] Charlesworth B. Driving genes and chromosomes[J]. Nature, 1988, 332（6163）: 394.

[18] [美]J萨姆布鲁克，DW拉塞尔. 分子克隆实验指南（上册）：3版[M]. 北京：科学出版社，2005：387-396

[19] Pham J L, Glaszmann J C, Sano R, et al. Isozyme markers in rice: genetic analysis and linkage relationships[J]. Genome, 1990, 33（3）: 348-359.

[20] Xu Y, Zhu L, Xiao J, et al. Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, doubled haploid, and recombinant inbred populations in rice （Oryza sativa L.)[J]. Mol Gen Genet, 1997, 253（5）: 535-545.

[21] Song X, Wang K, Guo W, et al. A comparison of genetic maps constructed from haploid and BC1 mapping populations from the same crossing between Gossypium hirsutum L and Gossypium barbadense L [J]. Genome, 2005, 48（3）: 378-390.

[22] 张德水，董伟，惠东威，等. 用栽培大豆与半野生大豆间的杂种 F2 群体构建基因组分子标记连锁框架图[J]. 科学通报，1997，42（12）：1326-1330.

[23] Kinshita T. Report of the committee on gene symbolization, nomenclature and linkage group[J]. Rice Genet Newl, 1991, 8：7939.

[24] Kinshita T. Report of the committee on gene symbolization, nomenclature and linkage group[J]. Rice Genet Newl, 1993, 8：7939.

[25] 彭勇，梁永书，王世全，等. 水稻 SSR 标记在 RI 群体的偏分离分析[J]. 分子植物育种，2006，4（6）：786-790.

[26] Zhao B, Deng Q M, Zhang Q J, et al. Analysis of segregation distortion of molecular markers in F2 population of rice[J]. Acta Genetica Sinica, 2006, 33（5）: 449-457.

[27] Matsushita S, Iseki T, Fukuta Y, et al. Characterization of segregation distortion on chromosome 3 induced in wide hybridization between indica and japonica type rice varieties[J]. Euphytica, 2003, 134（1）：27-32.

[28] Sibov S T, Lopes De Souza Jr, Garcia A A F, et al. Molecular mapping in tropical maize （Zea mays L.） using microsatellite markers. 2. Quantitative trait loci （QTL） for grain yield, plant heigth, ear height and grain moisture[J]. Hereditas, 2003, 139（2）: 107-115.

[29] Perfectti F, Pascual L. Segregation distortion of isozyme loci in cherimoya （Annona cherimola Mill）[J]. Theor Appl Genet, 1996, 93（3）: 440-446.

[30] 严建兵，汤华，黄益勤，等. 玉米 F2 群体分子标记偏分离的遗传分析[J]. 遗传学报，2003，30（10）：913-918.

[31] Sibov S T, Lopes De Souza Jr, Garcia A A F, et al. Molecular mapping in tropical maize （Zea mays L.） using microsatellite markers. 1. Map construction and localization of loci showing distorted segregation[J]. Hereditas, 2003, 139（2）: 96-106.

[32] Cloutier S, Cappadocia M, Landry B S. Analysis of RFLP mapping inaccuracy in Brassica napus L[J]. Theor Appl Genet, 1997, 95（1-2）: 83-91.